Difference between revisions of "Part:BBa K2558201:Design"

 
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===Design Notes===
 
===Design Notes===
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dCas9 sequence is modified with mutation A1341T in order to be RFC10 compatible.
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Sequence is modified at A1403T in order to be RFC10 compatible.
  
  
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===References===
 
===References===
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Gilbert LA et al. Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell. 2014 Oct 23;159(3):647-61.

Latest revision as of 08:12, 2 October 2018


dCas9 generator with Anderson weak promotor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1163
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3442
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequence is modified at A1403T in order to be RFC10 compatible.


Source

The sequence came from the registry and a commonly used CRISPRi system from Chen Lab.

References

Gilbert LA et al. Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation. Cell. 2014 Oct 23;159(3):647-61.