Difference between revisions of "Part:BBa K2609005:Design"
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===Source=== | ===Source=== | ||
| − | T4 bacteriophage genome, sequence from GenBank | + | T4 bacteriophage genome, sequence from GenBank: https://www.ncbi.nlm.nih.gov/nuccore/HM137666 |
===References=== | ===References=== | ||
| + | [1] Leiman, Petr G., et al. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host." Cell 118.4 (2004): 419-429.<br> | ||
| + | [2] Yoichi, Masatoshi, et al. "Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157: H7." Journal of biotechnology 115.1 (2005): 101-107.<br> | ||
| + | [3] Rakhuba, D. V., et al. "Bacteriophage receptors, mechanisms of phage adsorption and penetration into host cell." Pol. J. Microbiol 59.3 (2010): 145-155.<br> | ||
| + | [4] Bartual, Sergio G., et al. "Structure of the bacteriophage T4 long tail fiber receptor-binding tip." Proceedings of the National Academy of Sciences 107.47 (2010): 20287-20292. | ||
Latest revision as of 05:43, 2 October 2018
T4 bacteriophage long tail fiber protein gp37 (Modification IV)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 424
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence was codon optimized for expression in E. coli. The following changes were made to the wild-type protein sequence. ____
A double stop codon is placed at the end to ensure proper termination.
Source
T4 bacteriophage genome, sequence from GenBank: https://www.ncbi.nlm.nih.gov/nuccore/HM137666
References
[1] Leiman, Petr G., et al. "Three-dimensional rearrangement of proteins in the tail of bacteriophage T4 on infection of its host." Cell 118.4 (2004): 419-429.
[2] Yoichi, Masatoshi, et al. "Alteration of tail fiber protein gp38 enables T2 phage to infect Escherichia coli O157: H7." Journal of biotechnology 115.1 (2005): 101-107.
[3] Rakhuba, D. V., et al. "Bacteriophage receptors, mechanisms of phage adsorption and penetration into host cell." Pol. J. Microbiol 59.3 (2010): 145-155.
[4] Bartual, Sergio G., et al. "Structure of the bacteriophage T4 long tail fiber receptor-binding tip." Proceedings of the National Academy of Sciences 107.47 (2010): 20287-20292.