Difference between revisions of "Part:BBa K2623014"
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<partinfo>BBa_K2623014 short</partinfo> | <partinfo>BBa_K2623014 short</partinfo> | ||
| − | + | ===SUMMARY=== | |
| − | This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities. | + | This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.<br> |
| − | CAHS is a cytoplasmic-abundant protein which can strength the bacteria stress tolerance under the conditions such as dehydration. So taking biosafety into account, we add a constant promoter(BBa_J23100) into the CAHS circuit to express Lac I(BBa_S0100) in order to inhabit the initiation of the Lac O(BBa_R0011) promoter which can only be activated and then expressing CAHS and mRFP(BBa_J04650) after IPTG is added. | + | CAHS is a cytoplasmic-abundant protein which can strength the bacteria stress tolerance under the conditions such as dehydration. So taking biosafety into account, we add a constant promoter(BBa_J23100) into the CAHS circuit to express Lac I(BBa_S0100) in order to inhabit the initiation of the Lac O(BBa_R0011) promoter which can only be activated and then expressing CAHS and mRFP(BBa_J04650) after IPTG is added.<br> |
The separate fragment of CAHS is 714bp, and the whole circuit is 2934bp. | The separate fragment of CAHS is 714bp, and the whole circuit is 2934bp. | ||
Revision as of 14:49, 1 October 2018
Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator)
SUMMARY
This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acting as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.
CAHS is a cytoplasmic-abundant protein which can strength the bacteria stress tolerance under the conditions such as dehydration. So taking biosafety into account, we add a constant promoter(BBa_J23100) into the CAHS circuit to express Lac I(BBa_S0100) in order to inhabit the initiation of the Lac O(BBa_R0011) promoter which can only be activated and then expressing CAHS and mRFP(BBa_J04650) after IPTG is added.
The separate fragment of CAHS is 714bp, and the whole circuit is 2934bp.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 375
Illegal NheI site found at 1679
Illegal NheI site found at 1702 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2880
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1376
Illegal AgeI site found at 1488 - 1000COMPATIBLE WITH RFC[1000]