Difference between revisions of "Part:BBa K2623005"

(Structure of CikA)
(CikA's function in phosphorylation pathway)
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===CikA's function in phosphorylation pathway===
 
===CikA's function in phosphorylation pathway===
https://static.igem.org/mediawiki/parts/2/2c/PKaiBC_RpaA_.gif
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[[Image:PKaiBC_RpaA_.gif|thumb|200px|Fig.2]]
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===Usage and Biology===
 
===Usage and Biology===

Revision as of 13:32, 1 October 2018


CikA coding region

SUMMARY

It is a component of the Kai circuit, which dephosphorylates the phosphorylated RpaA, preventing phosphorylated RpaA from continually activating transcription of related promoters.
A separate fragment is 2265bp.

Structure of CikA

CikA.png

CikA's function in phosphorylation pathway

Fig.2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 127
    Illegal BamHI site found at 2187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]