Difference between revisions of "Part:BBa K2623005"

(Structure of CikA)
(Structure of CikA)
Line 9: Line 9:
 
https://static.igem.org/mediawiki/parts/7/75/CikA.png
 
https://static.igem.org/mediawiki/parts/7/75/CikA.png
  
 
+
===CikA's function in phosphorylation pathway===
 +
https://static.igem.org/mediawiki/parts/2/2c/PKaiBC_RpaA_.gif
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 13:30, 1 October 2018


CikA coding region

SUMMARY

It is a component of the Kai circuit, which dephosphorylates the phosphorylated RpaA, preventing phosphorylated RpaA from continually activating transcription of related promoters.
A separate fragment is 2265bp.

Structure of CikA

CikA.png

CikA's function in phosphorylation pathway

PKaiBC_RpaA_.gif Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 127
    Illegal BamHI site found at 2187
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]