Difference between revisions of "Part:BBa K2556000"
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<p>Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. Apart from its original function in bacterial immunity, the Cas9 protein has been heavily utilized as a genome engineering tool to induce site-directed double strand breaks in DNA. In this part, Cas9's expression can be induced by Ara. Upstream of cas is gfp, which can be used to indicate whether gene is normally transcribed.</p>
 
<p>Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. Apart from its original function in bacterial immunity, the Cas9 protein has been heavily utilized as a genome engineering tool to induce site-directed double strand breaks in DNA. In this part, Cas9's expression can be induced by Ara. Upstream of cas is gfp, which can be used to indicate whether gene is normally transcribed.</p>
 
<p>
 
<p>
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===Experimental approach===
 
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Revision as of 06:19, 1 October 2018


AraC-Pbad-gfp-Cas9

Cas9 is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes. Apart from its original function in bacterial immunity, the Cas9 protein has been heavily utilized as a genome engineering tool to induce site-directed double strand breaks in DNA. In this part, Cas9's expression can be induced by Ara. Upstream of cas is gfp, which can be used to indicate whether gene is normally transcribed.

Experimental approach

1

2

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1252
    Illegal NheI site found at 3092
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1771
    Illegal BamHI site found at 5371
    Illegal XhoI site found at 1672
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961