Difference between revisions of "Part:BBa K2856001:Design"
| (3 intermediate revisions by the same user not shown) | |||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | Sequence has been optimized for expression in Lactococcus lactis. We remove some illegal restriction sites in original squence and use ATG as | |
| − | + | initiation codon. | |
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | The original complete CDS sequence can be obtained from Genebank. Genebank accession: AP018935.1 | |
===References=== | ===References=== | ||
Latest revision as of 02:39, 1 October 2018
Bifunctional glutamate-cysteine ligase/glutathione synthase (gshF)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence has been optimized for expression in Lactococcus lactis. We remove some illegal restriction sites in original squence and use ATG as initiation codon.
Source
The original complete CDS sequence can be obtained from Genebank. Genebank accession: AP018935.1