Difference between revisions of "Part:BBa K2669000:Experience"
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===Applications of BBa_K2669000===
 
===Applications of BBa_K2669000===
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After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells.  Please note: The exact procedure can be found  <a href="http://2018.igem.org/wiki/index.php?title=Team:Uppsala/UnaG"><b>here</b></a> Bilirubin tests (addition of a small amount of bilirubin to samples) allowed us to see if the UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin.  </p>
  
 
"https://static.igem.org/mediawiki/2018/2/20/T--Uppsala--UnaG_Comparison.png"
 
"https://static.igem.org/mediawiki/2018/2/20/T--Uppsala--UnaG_Comparison.png"
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<p><b>Figure 2:</b> Bilirubin test before/after affinity chromatography.  Going from right to left the samples are:</p>
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                            <ul>
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                                <li> Lysed sample of the “bad” part before AC</li>
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                                <li> Lysed sample of the “good” part before AC</li>
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                                <li> "Bad" part after AC</li>
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                                <li> "Good" part after AC</li>
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                            </ul>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 11:38, 30 September 2018


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K2669000

After transforming our cells with a low copy amplicilin plasmid containing this composite part, cell lysis and affinity chromotography were used to extract UnaG from our cells. Please note: The exact procedure can be found <a href="http://2018.igem.org/wiki/index.php?title=Team:Uppsala/UnaG">here</a> Bilirubin tests (addition of a small amount of bilirubin to samples) allowed us to see if the UnaG was present in our samples, since as mentioned earlier UnaG fluoresces in the presence of bilirubin. </p>

"T--Uppsala--UnaG_Comparison.png"

Figure 2: Bilirubin test before/after affinity chromatography. Going from right to left the samples are:

  • Lysed sample of the “bad” part before AC
  • Lysed sample of the “good” part before AC
  • "Bad" part after AC
  • "Good" part after AC

User Reviews

UNIQ2ee3de8ff4e7dc6c-partinfo-00000000-QINU UNIQ2ee3de8ff4e7dc6c-partinfo-00000001-QINU