Difference between revisions of "Part:BBa K2623003"

(Structure of KaiC)
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[[Image:KaiC_z.png|thumb|300px|Fig.1]]</th><th>
 
[[Image:KaiC_z.png|thumb|300px|Fig.1]]</th><th>
[[Image:KaiC_x.png|thumb|300px|Fig.2]]</th><th>
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[[Image:KaiC_x.png|thumb|300px|Fig.2]]</th><th></table
[[Image:KaiC_1_.gif|thumb|500px|Fig.3]]</th><th></table
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[[Image:KaiC_1_.gif|thumb|500px|Fig.3]]<br>
 
===KaiC's phosphorylation stage===
 
===KaiC's phosphorylation stage===
 
https://static.igem.org/mediawiki/parts/d/d0/Kai_1_.gif <br>
 
https://static.igem.org/mediawiki/parts/d/d0/Kai_1_.gif <br>

Revision as of 08:30, 30 September 2018


KaiC coding region

SUMMARY

It is the core part of the Kai circuit, acting as a hexamer. This part can be both auto phosphorylated and dephosphorylated, and it can achieve about 24 hours of phosphorylation-dephosphorylation oscillation under the regulation of KaiA and KaiB.
Each KaiC monomer has two subunits, N-terminal CI subunit and C-terminal CII subunit. Each CII subunit has 431 serine (S431) residue and 432 threonine (T432) residue phosphorylation sites. Therefore, KaiC monomer has four possible phosphorylation states, showing different functions. SasA proteins will affect downstream signaling pathways, when it is phosphorylated by KaiC hexamers in a specific phosphorylated state. A separate fragment is 1560bp.

Structure of KaiC

Fig.1
Fig.2
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Fig.3

KaiC's phosphorylation stage

Kai_1_.gif
Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 148
    Illegal AgeI site found at 859
    Illegal AgeI site found at 868
    Illegal AgeI site found at 874
    Illegal AgeI site found at 1198
    Illegal AgeI site found at 1225
  • 1000
    COMPATIBLE WITH RFC[1000]