Difference between revisions of "Part:BBa K2865002"
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<partinfo>BBa_K2865002 short</partinfo> | <partinfo>BBa_K2865002 short</partinfo> | ||
− | The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle plasmids. They are named so because of their symmetry, which | + | The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle plasmids. They are named so because of their symmetry, which has been shown to be required for efficient multiplication of the AAV. We extracted both left and right ITRs through PCR and ligated them to each side of AAV-9 shuttle plasmid respectively. AAV-9 virus particles were then packaged and applied in therapeutic use. This part (BBa_K2865002) encodes for the left ITR of AAV-9. |
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Revision as of 15:14, 29 September 2018
[AAV9]-Left-ITR
The Inverted Terminal Repeats (ITRs) are two 145-base sequences that locate on both sides of recombinant AAV (rAAV) shuttle plasmids. They are named so because of their symmetry, which has been shown to be required for efficient multiplication of the AAV. We extracted both left and right ITRs through PCR and ligated them to each side of AAV-9 shuttle plasmid respectively. AAV-9 virus particles were then packaged and applied in therapeutic use. This part (BBa_K2865002) encodes for the left ITR of AAV-9.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25INCOMPATIBLE WITH RFC[25]Unknown
- 1000COMPATIBLE WITH RFC[1000]