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Revision as of 10:07, 28 September 2018


sfGFP Coding Sequence

Part BBa_I746916 standarized as Phytobrick. Super Folder Green Fluorescent Protein. It can be used to assemble transcriptional units with the Golden Gate assembly method, and also, it can be used as a Biobrick.

We have obtained the emission and excitation spectrum of this protein following this protocol and with these parameters:

Parameter Value
Minimum excitation wavelenght 0
Maximum excitation wavelenght 0
Emission wavelenght 0
Minimum emission wavelenght 0
Maximun emission wavelenght 0
Excitation wavelenght 0
G 0


This is the spectrum of sfGFP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 14