Difference between revisions of "Part:BBa K2669001"

 
Line 1: Line 1:
 +
__NOTOC__
  
 +
<partinfo>BBa_K2669001 short</partinfo>
 +
 +
 +
WORK IN PROGRESS
 +
 +
This part is a correction of the Uppsala 2016’s [[Part:BBa_K2003011]], which we noticed had a misplaced start codon. This misplaced start codon caused two major problems:
 +
1) This would lead to a reduction in UnaG expression due to a large space being created between the RBS and the start codon
 +
2) This would lead to no histidine tag being expressed
 +
 +
We have shown that our new improved part does in fact express a histidine tag and has higher expression levels than the old part [[Part:BBa_K2003011]]. 
 +
 +
UnaG is a unique chromoprotein since it expresses fluorescent signal when in contact with bilirubin. This means (unlike most other chromoproteins) it can be used as a reporter in anaerobic environments, or potentially in environments where bilirubin is naturally present, such as the intestines.
 +
 +
 +
<!-- Add more about the biology of this part here
 +
===Usage and Biology===
 +
 +
<!-- -->
 +
<span class='h3bb'>Sequence and Features</span>
 +
<partinfo>BBa_K2669000 SequenceAndFeatures</partinfo>
 +
 +
 +
<!-- Uncomment this to enable Functional Parameter display
 +
===Functional Parameters===
 +
<partinfo>BBa_K2669000 parameters</partinfo>
 +
<!-- -->

Revision as of 10:12, 27 September 2018


Bilirubin-inducible UnaG+6xHis-tag+Flexible linker


WORK IN PROGRESS

This part is a correction of the Uppsala 2016’s Part:BBa_K2003011, which we noticed had a misplaced start codon. This misplaced start codon caused two major problems: 1) This would lead to a reduction in UnaG expression due to a large space being created between the RBS and the start codon 2) This would lead to no histidine tag being expressed

We have shown that our new improved part does in fact express a histidine tag and has higher expression levels than the old part Part:BBa_K2003011.

UnaG is a unique chromoprotein since it expresses fluorescent signal when in contact with bilirubin. This means (unlike most other chromoproteins) it can be used as a reporter in anaerobic environments, or potentially in environments where bilirubin is naturally present, such as the intestines.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]