Difference between revisions of "Part:BBa K2721002"
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We put the Biobrick E0040(GFP) behind the K2721000 and R0085 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2721000 and wild-type T7 promoter(R0085) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below. | We put the Biobrick E0040(GFP) behind the K2721000 and R0085 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2721000 and wild-type T7 promoter(R0085) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below. | ||
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According to the data, it is easy to find that the ratio of relative strength of K2721000 and R0085 is 7.63, indicating that this part may show much higher expression efficiency relative to the WT-T7 promoter. | According to the data, it is easy to find that the ratio of relative strength of K2721000 and R0085 is 7.63, indicating that this part may show much higher expression efficiency relative to the WT-T7 promoter. | ||
Latest revision as of 02:32, 27 September 2018
Modified T7 promoter 3
BBa_K2721002
Modified Promoter T7
This part contains a T7 promoter derived from BBa_R0085 with some point mutation. To express BioBricks under the control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene.
Usage and Biology
We put the Biobrick E0040(GFP) behind the K2721000 and R0085 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2721000 and wild-type T7 promoter(R0085) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below.
According to the data, it is easy to find that the ratio of relative strength of K2721000 and R0085 is 7.63, indicating that this part may show much higher expression efficiency relative to the WT-T7 promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]