Difference between revisions of "Part:BBa K2810011"

Revision as of 10:23, 26 September 2018

mCherry under control of the bacterial promoter and terminator

mCherry, codon optimised for Nicotiana benthamiana, under control of part BBa_J23100 (bacterial promoter) and BBa_B0015 (bacterial terminator). The RBS is part BBa_B0030 between the promoter and CDS. This part was used to show that the coding sequence of our mCherry part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2810009">(BBa_K2810009)</a> was fully functional. The plasmid it is in (pSB1C3) encodes a chloramphenicol resistance gene.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2810011 short</partinfo>
-mCherry, codon optimised for <i>Nicotiana benthamiana</i>, under control of the X bacterial promoter and X bacterial terminator. This part was used to show that the coding sequence of our mCherry part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2810009">(BBa_K2810009)</a> was fully functional. The plasmid it is in (pSB1C3) encodes a chloramphenicol resistance gene.
+mCherry, codon optimised for <i>Nicotiana benthamiana</i>, under control of part BBa_J23100 (bacterial promoter) and BBa_B0015  (bacterial terminator). The RBS is part BBa_B0030 between the promoter and CDS. This part was used to show that the coding sequence of our mCherry part <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2810009">(BBa_K2810009)</a> was fully functional. The plasmid it is in (pSB1C3) encodes a chloramphenicol resistance gene.
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