Difference between revisions of "Part:BBa K2599006"

Revision as of 11:58, 25 September 2018

Antimicrobial peptide - Leucocyclicin Q

This biobrick is the basic part of the Leucocyclicin Q, more information please look for the composite part [1].

Figure 1. Basic part of Leucocyclicin Q

Introduction

Leucocyclicin Q, produced by Leuconostoc mesenteroides TK41401, possesses antimicrobial activity against broad range of bacteria srains, and particularly shows strong activity against Bacillus coagulans. Leucocyclicin Q was proved to be a cyclic bacteriocin in which N and C termini are bound to each other, and its structure is very similar to Lactocyclicin Q. Furthermore, it shows high stability against different pH value or heat stress.

Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Leucocyclicin Q sequence length is around 186 b.p.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2599006 short</partinfo>
-.
+This biobrick is the basic part of the Leucocyclicin Q, more information please look for the composite part [https://parts.igem.org/Part:BBa_K2599014(BBa_K2599014)].
+{{#tag:html|<img style="width: 20%; padding-left: 30%;" src="https://static.igem.org/mediawiki/2018/d/d5/T--NCTU_Formosa--Leu.png" alt="" />}}
+<div style="width:40%; padding-left: 20%;"><p style="padding-top: 10px; font-size: 10px; text-align: center;"><b>Figure 1.</b> Basic part of Leucocyclicin Q</p></div>
+<p style="padding-top:20px;font-size:20px"><b>Introduction</b></p>
+Leucocyclicin Q, produced by <i>Leuconostoc mesenteroides</i> TK41401, possesses antimicrobial activity against broad range of bacteria srains, and particularly shows strong activity against <i>Bacillus coagulans</i>. Leucocyclicin Q was proved to be a cyclic bacteriocin in which N and C termini are bound to each other, and its structure is very similar to Lactocyclicin Q. Furthermore, it shows high stability against different pH value or heat stress.
+<p style="padding-top:10px;font-size:20px;"><b>Experiment Result</b></p>
+===Cloning===
+We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Leucocyclicin Q sequence length is around 186 b.p.
 <!-- Add more about the biology of this part here
@@ Line 17: / Line 36: @@
 <partinfo>BBa_K2599006 parameters</partinfo>
 <!-- -->
+<p style="padding-top:20px;font-size:20px"><b>Reference</b></p>