Difference between revisions of "Part:BBa K2599005"

 
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This biobrick is the basic part of the Lacticin Z, more information please look for the composite part [https://parts.igem.org/Part:BBa_K2599013(BBa_K2599013)].
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{{#tag:html|<img style="width: 20%; padding-left: 30%;" src="https://static.igem.org/mediawiki/2018/3/35/T--NCTU_Formosa--Ent_96.png" alt="" />}}
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<div style="width:40%; padding-left: 20%;"><p style="padding-top: 10px; font-size: 10px; text-align: center;"><b>Figure 1.</b> Basic part of Enterocin 96</p></div>
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<p style="padding-top:20px;font-size:20px"><b>Introduction</b></p>
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Lacticin Z, produced by <i>Lactoccus lactis</i> QU 14, has no leader sequence or signal peptide. Also, it exhibits a nanomolar range of MICs against various gram-positve bacteria. Consequently, Lacticin Z indeed shows the best antibacterial activity in our experiment.
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<p style="padding-top:10px;font-size:20px;"><b>Experiment Result</b></p>
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===Cloning===
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We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Lacticin Z sequence length is around 153 b.p.
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<partinfo>BBa_K2599005 parameters</partinfo>
 
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<p style="padding-top:20px;font-size:20px"><b>Reference</b></p>

Revision as of 11:54, 25 September 2018


Antimicrobial peptide - Lacticin Z

This biobrick is the basic part of the Lacticin Z, more information please look for the composite part [1].



Figure 1. Basic part of Enterocin 96


Introduction

Lacticin Z, produced by Lactoccus lactis QU 14, has no leader sequence or signal peptide. Also, it exhibits a nanomolar range of MICs against various gram-positve bacteria. Consequently, Lacticin Z indeed shows the best antibacterial activity in our experiment.


Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Lacticin Z sequence length is around 153 b.p.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference