Difference between revisions of "Part:BBa K2599003"
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This biobrick is the basic part of the enterocin A, more information please look for the composite part [https://parts.igem.org/Part:BBa_K2599011(BBa_K2599011)].
 
This biobrick is the basic part of the enterocin A, more information please look for the composite part [https://parts.igem.org/Part:BBa_K2599011(BBa_K2599011)].
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{{#tag:html|<img style="width: 20%; padding-left: 30%;" src="https://static.igem.org/mediawiki/2018/4/4a/T--NCTU_Formosa--Ent_B.png" alt="" />}}
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<div style="width:40%; padding-left: 20%;"><p style="padding-top: 10px; font-size: 10px; text-align: center;"><b>Figure 1.</b> Basic part of Enterocin B</p></div>
  
  

Revision as of 11:38, 25 September 2018


Antimicrobial peptide - Enterocin B

This biobrick is the basic part of the enterocin A, more information please look for the composite part [1].



Figure 1. Basic part of Enterocin B


Introduction

Same as enterocin A, enterocin B is also from Enterococcus faecium. However, enterocin B does not belong to the pediocin family of bacteriocins. Enterocin B is found to be a heat-stable bacteriocins, which can synergistically act with enterocin A.


Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The enterocin B sequence length is around 210 b.p.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Reference