Difference between revisions of "Part:BBa K2871000:Design"

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===References===
 
===References===
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DOI: 10.1128/JB.186.16.5486-5495.2004 https://jb.asm.org/content/186/16/5486
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Charpentier & Oswald, 2004. journal of baceteriology. 'Identification of the Secretion and Translocation Domain of the Enteropathogenic and Enterohemorrhagic Escherichia coli Effector Cif, Using TEM-1 β-Lactamase as a New Fluorescence-Based Reporter' DOI: 10.1128/JB.186.16.5486-5495.2004  
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https://jb.asm.org/content/186/16/5486

Revision as of 20:25, 24 September 2018


Map20, T3SS export signal peptide from Map gene


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 85
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The paper described by Charpentier & Oswald (2004), showed that a 20 amino acid sequence was sufficient albeit not the best. The short length however seemed attractive to us, because we wanted to minimize folding interference between the signal sequence and the intended protein fused to the signal sequence.


Source

Synthetic sequence deduced from Amino acid Sequence from the Enteropathogenic E. coli strain E22. Described in the paper Charpentier & Oswald, 2004. 'Identification of the secretion and translocation domain of the enteropathogenic end enterohemorrhagic escherichia coli effector cif, using TEM-1-beta-lactamase as a new fluorescence-Based Reporter'

References

Charpentier & Oswald, 2004. journal of baceteriology. 'Identification of the Secretion and Translocation Domain of the Enteropathogenic and Enterohemorrhagic Escherichia coli Effector Cif, Using TEM-1 β-Lactamase as a New Fluorescence-Based Reporter' DOI: 10.1128/JB.186.16.5486-5495.2004 https://jb.asm.org/content/186/16/5486