Difference between revisions of "Part:BBa K2629001:Experience"
| Line 2: | Line 2: | ||
<p>Experiments were done on a plasmid in which the probe has been inserted, thanks the Gibson technic, in psB1C3-BBa_J04450. </p> | <p>Experiments were done on a plasmid in which the probe has been inserted, thanks the Gibson technic, in psB1C3-BBa_J04450. </p> | ||
| + | |||
| + | <h1> Cloning results </h1> | ||
| + | |||
| + | Sequencage | ||
<h1> Test A: <I> Is this part able to detect the target for which it has been designed ?</I> </h1><br> | <h1> Test A: <I> Is this part able to detect the target for which it has been designed ?</I> </h1><br> | ||
Revision as of 12:55, 23 September 2018
Experiments were done on a plasmid in which the probe has been inserted, thanks the Gibson technic, in psB1C3-BBa_J04450.
Contents
Cloning results
Sequencage
Test A: Is this part able to detect the target for which it has been designed ?
mettre schema
Test B: Is this part able to detect specifically the target for which it has been designed ?
In order to evaluate the specificity of the detector part of BBa_K2629001, different “false target” sequences have been synthesized and tested with the detector.
An algorithm was made by iGEM Grenoble Alpes 2017 to give random sequences, more or less homologous to the original target with 5%, 15%, 25% and 50% randomly modified pairs of nucleotides (length is kept 36bp).
The algorithm can be found here :
These 50%, 75%, 85%, 95% homologous sequences to the target were tested 6 times. Unfortunately, results were too heterogenous to bring any conclusions, notably about the number of false positive CFU (there is a possibility that the detector was badly digested and was consequently transformed) or the supposedly increasing number of colonies. Indeed, transformation includes lot of steps with specific parameters, that all vary a little between each experiment and may explain the lack of reproducibility.
User Reviews
UNIQeb7e4db3869f230b-partinfo-00000000-QINU UNIQeb7e4db3869f230b-partinfo-00000001-QINU