Difference between revisions of "Part:BBa K2599003"

Revision as of 17:21, 20 September 2018

Antimicrobial peptide - Enterocin B

This biobrick is the basic part of the enterocin A, more information please look for the composite part [1].

Introduction

Same as enterocin A, enterocin B is also from Enterococcus faecium. However, enterocin B does not belong to the pediocin family of bacteriocins. Enterocin B is found to be a heat-stable bacteriocins, which can synergistically act with enterocin A.

Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The enterocin B sequence length is around 210 b.p.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Reference

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2599003 short</partinfo>
-.
+This biobrick is the basic part of the enterocin A, more information please look for the composite part [https://parts.igem.org/Part:BBa_K2599011(BBa_K2599011)].
+<p style="padding-top:20px;font-size:20px"><b>Introduction</b></p>
+Same as [https://parts.igem.org/Part:BBa_K2599010 enterocin A], enterocin B is also from <i>Enterococcus faecium</i>. However, enterocin B does not belong to the pediocin family of bacteriocins. Enterocin B is found to be a heat-stable bacteriocins, which can synergistically act with enterocin A.
+<p style="padding-top:10px;font-size:20px;"><b>Experiment Result</b></p>
+===Cloning===
+We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The enterocin B sequence length is around 210 b.p.
 <!-- Add more about the biology of this part here
@@ Line 11: / Line 25: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2599003 SequenceAndFeatures</partinfo>
+<p style="padding-top:20px;font-size:20px"><b>Reference</b></p>
 <!-- Uncomment this to enable Functional Parameter display