Difference between revisions of "Part:BBa K2599001"
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Bovicin HJ50 is isolated from <i> Streptococcus bovis </i> HJ50. It contains a disulfide bridge and shows similarity to type AII lantibiotics, the largest group of lantibiotics. Like most of the bacteriocins produced by lactic acid bacteria, Bovicin HJ50 showed a narrow range of inhibiting activity. Its antimicrobial activity has been proved in reference. | Bovicin HJ50 is isolated from <i> Streptococcus bovis </i> HJ50. It contains a disulfide bridge and shows similarity to type AII lantibiotics, the largest group of lantibiotics. Like most of the bacteriocins produced by lactic acid bacteria, Bovicin HJ50 showed a narrow range of inhibiting activity. Its antimicrobial activity has been proved in reference. | ||
Revision as of 13:48, 19 September 2018
Antimicrobial peptide - Bovicin HJ50
This biobrick is the basic part of the Bovicin HJ50, more information please look for the composite part (BBa_K2599009).
Figure 1 biobrick picture
Introduction
Bovicin HJ50 is isolated from Streptococcus bovis HJ50. It contains a disulfide bridge and shows similarity to type AII lantibiotics, the largest group of lantibiotics. Like most of the bacteriocins produced by lactic acid bacteria, Bovicin HJ50 showed a narrow range of inhibiting activity. Its antimicrobial activity has been proved in reference.
Experiment Result
Cloning
We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Bovicin HJ50 sequence length is around 171 b.p.
Figure 2 PCR
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 20
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference