Difference between revisions of "Part:BBa K2599001"

 
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<partinfo>BBa_K2599001 short</partinfo>
 
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This biobrick is the basic part of the Bovicin HJ50, more information please look for the composite part [https://parts.igem.org/Part:BBa_K2599009 (BBa_K2599009)].
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Figure 1 biobrick picture
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p style="padding-top:20px;font-size:20px"><b>Introduction</b></p>
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Bovicin HJ50 is isolated from <i> Streptococcus bovis </i> HJ50. It contains a disulfide bridge and shows similarity to type AII lantibiotics, the largest group of lantibiotics. Like most of the bacteriocins produced by lactic acid bacteria, Bovicin HJ50 showed a narrow range of inhibiting activity. Its antimicrobial activity has been proved in reference.
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<p style="padding-top:10px;font-size:20px;"><b>Experiment Result</b></p>
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===Cloning===
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We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Bovicin HJ50 sequence length is around 171 b.p.
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Figure 2 PCR
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<partinfo>BBa_K2599001 parameters</partinfo>
 
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<p style="padding-top:20px;font-size:20px"><b>Reference</b></p>

Revision as of 13:48, 19 September 2018


Antimicrobial peptide - Bovicin HJ50

This biobrick is the basic part of the Bovicin HJ50, more information please look for the composite part (BBa_K2599009).


Figure 1 biobrick picture

p style="padding-top:20px;font-size:20px">Introduction</p>

Bovicin HJ50 is isolated from Streptococcus bovis HJ50. It contains a disulfide bridge and shows similarity to type AII lantibiotics, the largest group of lantibiotics. Like most of the bacteriocins produced by lactic acid bacteria, Bovicin HJ50 showed a narrow range of inhibiting activity. Its antimicrobial activity has been proved in reference.


Experiment Result

Cloning

We conbined our toxic gene to pSB1C3 backbone and conducted PCR to check the size of our part. The Bovicin HJ50 sequence length is around 171 b.p.


Figure 2 PCR


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 20
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference