Difference between revisions of "Part:BBa K2810010"

 
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<partinfo>BBa_K2810010 short</partinfo>
 
<partinfo>BBa_K2810010 short</partinfo>
  
A promoter sequence that is activated by Ribitol, an ribose-derived compound. This part is GoldenGate compatible.
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A regulatory region derived from the ribitol-responsive operon in bacteria. This part is GoldenGate compatible.
  
 
The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.  
 
The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.  
  
The [http://2018.igem.org/Team:Cardiff_Wales Cardiff_Wales] team is working with this promotor part through a collaboration with [http://2018.igem.org/Team:WashU_StLouis Washington University iGEM].  
+
The [http://2018.igem.org/Team:Cardiff_Wales Cardiff_Wales] team is working with this regulatory sequence through a collaboration with [http://2018.igem.org/Team:WashU_StLouis Washington University iGEM].  
  
 
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Latest revision as of 12:04, 18 September 2018


Regulatory region derived from the ribitol-responsive operon

A regulatory region derived from the ribitol-responsive operon in bacteria. This part is GoldenGate compatible.

The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.

The [http://2018.igem.org/Team:Cardiff_Wales Cardiff_Wales] team is working with this regulatory sequence through a collaboration with [http://2018.igem.org/Team:WashU_StLouis Washington University iGEM].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 57
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]