Difference between revisions of "Part:BBa K2810010"

Latest revision as of 12:04, 18 September 2018

Regulatory region derived from the ribitol-responsive operon

A regulatory region derived from the ribitol-responsive operon in bacteria. This part is GoldenGate compatible.

The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.

The [http://2018.igem.org/Team:Cardiff_Wales Cardiff_Wales] team is working with this regulatory sequence through a collaboration with [http://2018.igem.org/Team:WashU_StLouis Washington University iGEM].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 57
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2810010 short</partinfo>
-A promoter sequence that is activated by Ribitol, an ribose-derived compound. This part is GoldenGate compatible.
+A regulatory region derived from the ribitol-responsive operon in bacteria. This part is GoldenGate compatible.
-The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that makes the sequence conforms to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.
+The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.
+The [http://2018.igem.org/Team:Cardiff_Wales Cardiff_Wales] team is working with this regulatory sequence through a collaboration with [http://2018.igem.org/Team:WashU_StLouis Washington University iGEM].
 <!-- Add more about the biology of this part here