Difference between revisions of "Part:BBa K2721003"

Revision as of 11:44, 13 September 2018

Modified T7 promoter 4

BBa_K2721003

Modified Promoter T7

This part contains a T7 promoter derived from BBa_R0085 with some point mutation. To express BioBricks under the control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene.

Usage and Biology

We put the Biobrick E0040(GFP) behind the K2721000 and R0085 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2721000 and wild-type T7 promoter(R0085) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below.

According to the data, it is easy to find that the ratio of relative strength of K2721000 and R0085 is 13.92, indicating that this part may show much higher expression efficiency relative to the WT-T7 promoter.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2721003 short</partinfo>
-This promoter is quite short. So we recommend PCR to assembly it with protein coding sequence.
+BBa_K2721003
+Modified Promoter T7
+This part contains a T7 promoter derived from <partinfo>R0085</partinfo> with some point mutation. To express BioBricks under the control of this promoter, similar to the wild-type T7 promoter, the E.coli chassis should carry a T7 polymerase gene.
+<b>Usage and Biology</b>
+We put the Biobrick E0040(GFP) behind the K2721000 and R0085 to construct the expression system so that we can compare the expression efficiency of WT-T7 promoter and the modified promoter. The relative strength of K2721000 and wild-type T7 promoter(R0085) is assessed by measuring the relative fluorescence unit after 4 hours of induction by IPTG in E.coli BL21(DE3). And the result is shown in the figure below.
+https://static.igem.org/mediawiki/2017/2/2a/ZJU_China_R_25.png
+According to the data, it is easy to find that the ratio of relative strength of K2721000 and R0085 is 13.92, indicating that this part may show much higher expression efficiency relative to the WT-T7 promoter.
 <!-- Add more about the biology of this part here