Difference between revisions of "Part:BBa K2586001:Design"

 
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===Design Notes===
 
===Design Notes===
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This part serves as foundation to the experiments of the iGEM Team Goettingen 2018 as it confers glyphosate resistance to Bacillus subtilis strains.
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This part was amplified from ''<i>Bacillus subtilis</i>'' genomic DNA and ligated into the ''Eco''RI and ''Pst''I digested '''pSB1C3''' backbone.
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===Source===
 
===Source===
  
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This part is a PCR product made through de novo synthesis in the lab.  
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This basic part consists a gene that was amplified by PCR using chromosomal DNA of the wild type strain ''B. subtilis''  168 (Laboratory collection AG Commichau, Department for General Microbiology, University Göttingen, Germany).
  
 
===References===
 
===References===
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[https://www.ncbi.nlm.nih.gov/pubmed/18723616 (Zeigler <i>et al.</i> 2008)]: The origins of 168, W23, and other <i>Bacillus subtilis</i> legacy strains. J. Bacteriol. 190: 6983-95.

Latest revision as of 11:04, 13 September 2018


GltT: glutamate and glyphosate transporter in B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.

Source

This basic part consists a gene that was amplified by PCR using chromosomal DNA of the wild type strain B. subtilis 168 (Laboratory collection AG Commichau, Department for General Microbiology, University Göttingen, Germany).

References

(Zeigler et al. 2008): The origins of 168, W23, and other Bacillus subtilis legacy strains. J. Bacteriol. 190: 6983-95.