Difference between revisions of "Part:BBa K2586019:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The sequence of the GAT-enzyme was ordered from Integrated DNA Technologies [https://eu.idtdna.com/pages (IDT)]. We used self designed primers to create the fitting ends and inserted the so prepared part as template into the <i>PstI</i> and <i>EcoRI</i> digested <b>pSB1C3</b> backbone. | |
===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| − | + | [https://www.ncbi.nlm.nih.gov/pubmed/15155947 (Castle <i>et. al.</i> 2004)]: Discovery and Directed Evolution of a Glyphosate Tolerance Gene | |
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Latest revision as of 11:00, 13 September 2018
GAT: Glyphosate N-Acetyltransferase
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The sequence of the GAT-enzyme was ordered from Integrated DNA Technologies (IDT). We used self designed primers to create the fitting ends and inserted the so prepared part as template into the PstI and EcoRI digested pSB1C3 backbone.
Source
This part was first described in: Castle et. al. paper (2004). We took this paper as base for the experimental process under the hypothesis that GAT could potentially be inserted into Bacillus subtilis to enable the bacteria to inactivate glyphosate.
References
(Castle et. al. 2004): Discovery and Directed Evolution of a Glyphosate Tolerance Gene