Difference between revisions of "Part:BBa K2586019:Design"

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===Design Notes===
 
===Design Notes===
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We inserted the ordered GAT-enzyme into the <i>PstI</i> and <i>EcoRI</i> digested <b>pSB1C3</b> backbone
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This part was ordered from Integrated DNA Technologies [https://eu.idtdna.com/pages (IDT)]. We inserted the ordered GAT-sequence as template into the <i>PstI</i> and <i>EcoRI</i> digested <b>pSB1C3</b> backbone.
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===Source===
 
===Source===

Revision as of 10:52, 13 September 2018


GAT: Glyphosate N-Acetyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was ordered from Integrated DNA Technologies (IDT). We inserted the ordered GAT-sequence as template into the PstI and EcoRI digested pSB1C3 backbone.

Source

This part was first described in: Castle et. al. paper (2004). We ordered the gene from IDT (Integrated DNA Technologies) and used it as insert for our experiments.

References

<a href="http://science.sciencemag.org/content/304/5674/1151">Castle et. al. 2004</a> (1) Castle et. al. 2004: Discovery and Directed Evolution of a Glyphosate Tolerance Gene