Difference between revisions of "Part:BBa K2586009:Design"
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===Source=== | ===Source=== | ||
| − | This part was made using [https://parts.igem.org/Part:BBa_K2586001 (BBa_K2586001)] and [https://parts.igem.org/Part:BBa_K2586007 (BBa_K2586007)] as templates to carry out a PCR. The PCR product was used as insert to clone this part into the required pSB1C3 backbone. | + | This part was made using [https://parts.igem.org/Part:BBa_K2586001 (BBa_K2586001)] and [https://parts.igem.org/Part:BBa_K2586007 (BBa_K2586007)] as templates to carry out a PCR. The PCR product was used as insert to clone this part into the required <i>EcoRI</i> and <i>PstI</i> digested <b>pSB1C3</b> backbone. |
===References=== | ===References=== | ||
Latest revision as of 08:27, 13 September 2018
RBS-gltT: Combining gltT with a RBS.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 125
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 125
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 125
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 125
Illegal NgoMIV site found at 761
Illegal NgoMIV site found at 1142 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Yet to come
Source
This part was made using (BBa_K2586001) and (BBa_K2586007) as templates to carry out a PCR. The PCR product was used as insert to clone this part into the required EcoRI and PstI digested pSB1C3 backbone.