Difference between revisions of "Part:BBa K2586009:Design"

 
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===Source===
 
===Source===
  
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This part was made using BBa_K2586001 and BBa_K2586007 as templates to carry out a PCR. The PCR product was used as insert to clone this part into the required pSB1C3 backbone.  
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This part was made using [https://parts.igem.org/Part:BBa_K2586001 (BBa_K2586001)] and [https://parts.igem.org/Part:BBa_K2586007 (BBa_K2586007)] as templates to carry out a PCR. The PCR product was used as insert to clone this part into the required <i>EcoRI</i> and <i>PstI</i> digested <b>pSB1C3</b> backbone.
  
 
===References===
 
===References===

Latest revision as of 08:27, 13 September 2018


RBS-gltT: Combining gltT with a RBS.


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 125
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 125
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 125
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 125
    Illegal NgoMIV site found at 761
    Illegal NgoMIV site found at 1142
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Yet to come


Source

This part was made using (BBa_K2586001) and (BBa_K2586007) as templates to carry out a PCR. The PCR product was used as insert to clone this part into the required EcoRI and PstI digested pSB1C3 backbone.

References