Difference between revisions of "Part:BBa K2586019:Design"

 
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===References===
 
===References===
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<a href="http://science.sciencemag.org/content/304/5674/1151">Castle <i> et. al. </i> 2004</a>
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(1) Castle <i>et. al.</i> 2004: Discovery and Directed Evolution of a Glyphosate Tolerance Gene

Revision as of 13:50, 11 September 2018


GAT: Glyphosate N-Acetyltransferase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We inserted the ordered GAT-enzyme into the PstI and EcoRI digested pSB1C3 backbone


Source

This part was first described in: Castle et. al. paper (2004). We ordered the gene from IDT (Integrated DNA Technologies) and used it as insert for our experiments.

References

<a href="http://science.sciencemag.org/content/304/5674/1151">Castle et. al. 2004</a> (1) Castle et. al. 2004: Discovery and Directed Evolution of a Glyphosate Tolerance Gene