Difference between revisions of "Part:BBa K2615007:Design"

(Design Notes)
(Source)
Line 12: Line 12:
  
 
===Source===
 
===Source===
 
+
<p>
From
+
Science Museum No.105,College of Marine Life Sciences, Ocean University of China
 +
</p>
  
 
===References===
 
===References===

Revision as of 12:44, 11 September 2018


Csy4-H29A, the No.5 member of Csy4 family.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 93


Design Notes

We designed this part by point mutation. We changed the CAC(encoding His ) to GCG(encoding Ara) on the 29th site based on wild type Csy4.

Source

Science Museum No.105,College of Marine Life Sciences, Ocean University of China

References