Difference between revisions of "Part:BBa K2615004:Design"

(→‎Design Notes)
(→‎Source)
Line 12: Line 12:
  
 
===Source===
 
===Source===
−
 
+
<p>
−
 
+
Science Museum No.105，College of Marine Life Sciences, Ocean University of China
−
  The cleavage of Cys4 releases a cis-repressive RNA module (crRNA,paired with RBS) from the masked ribosome binding site (RBS), which subsequently allows the downstream translation initiation. A Ribosome Binding Site (RBS) is an RNA sequence to which ribosomes can bind and initiate translation. Combined RBS and Cys4, it can be more convenient for other iGEMers to use this composite part without putting a RBS aequence on the upstream of Cys4 coding sequence.
+
</p>
  
 
===References===
 
===References===

Revision as of 12:43, 11 September 2018


Csy4-Q104A, the No.2 member of Csy4 family.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 377
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 93


Design Notes

We designed this part by point mutation. We changed the CAG(encoding Gln) to GCG(encoding Ala) on the 104th site based on wild type Csy4.

Source

Science Museum No.105，College of Marine Life Sciences, Ocean University of China

References