Difference between revisions of "Part:BBa K2615006:Design"
(→Design Notes) |
|||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | <p> | |
| − | + | We designed this part by point mutation. We changed the TTC(encoding Phe) to GCG(encoding Ara) on the 155th site based on wild type Csy4. | |
| − | + | </p> | |
===Source=== | ===Source=== | ||
Revision as of 12:36, 11 September 2018
Csy4-F155A, the No.4 member of Csy4 family.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 377
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 93
Design Notes
We designed this part by point mutation. We changed the TTC(encoding Phe) to GCG(encoding Ara) on the 155th site based on wild type Csy4.
Source
From