Difference between revisions of "Part:BBa K2586000"

 
 
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<partinfo>BBa_K2586000 short</partinfo>
 
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<i>Palf4</i> is an artificial promoter that is constitutively expressed in <i>Bacillus subtilis</i>. It has the advantage to have only a small DNA sequence of 30 bp length.  
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<i>Palf4</i> is an artificial promoter that is constitutively active in <i>Bacillus subtilis</i>. The part has the advantage that it consists of a short DNA sequence (30 bp).  
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The promoter sequence can be fused with <i>lacz</i> and a riboswitch, to carry out experiments with the characteristic enzyme &#946;-Galactosidase.
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The promoter sequence can be fused to reporter genes, such as <i>lacZ</i> as well as to any other gene.  
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Artificial promoter, which is consttuitevely active in B. subtilis. The advantage: Very short sequence. References: Gundlach et. Al.  
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===Usage and Biology===
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Latest revision as of 11:08, 11 September 2018


Palf4: Constitutively active promoter for Bacillus subtilis

Palf4 is an artificial promoter that is constitutively active in Bacillus subtilis. The part has the advantage that it consists of a short DNA sequence (30 bp). The promoter sequence can be fused to reporter genes, such as lacZ as well as to any other gene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]