Difference between revisions of "Part:BBa K2586000"

Revision as of 11:08, 11 September 2018

Palf4: Constitutively active promoter for Bacillus subtilis

Palf4 is an artificial promoter that is constitutively active in Bacillus subtilis. The part has the advantage that it consists of a short DNA sequence (30 bp). The promoter sequence can be fused to reporter genes, such as lacZ as well as to any other gene.

Artificial promoter, which is constuitevely active in B. subtilis. Its advantage lays in the short sequence, which allows it to be introduced into genetic systems with ease. Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2586000 short</partinfo>
-<i>Palf4</i> is an artificial promoter that is constitutively expressed in <i>Bacillus subtilis</i>. It has the advantage to have only a small DNA sequence of 30 bp length.
+<i>Palf4</i> is an artificial promoter that is constitutively active in <i>Bacillus subtilis</i>. The part has the advantage that it consists of a short DNA sequence (30 bp).
-The promoter sequence can be fused with <i>lacz</i> and a riboswitch, to carry out experiments with the characteristic enzyme &#946;-Galactosidase.
+The promoter sequence can be fused to reporter genes, such as <i>lacZ</i> as well as to any other gene.
-Artificial promoter, which is consttuitevely active in B. subtilis. Its advantage lays in the short sequence, which allows it to be introduced into genetic systems with ease.
+Artificial promoter, which is constuitevely active in B. subtilis. Its advantage lays in the short sequence, which allows it to be introduced into genetic systems with ease.
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