Difference between revisions of "Part:BBa K2586003"

Revision as of 10:19, 11 September 2018

aroE: 3-phosphoshikimate 1-carboxyvinyltransferase

This part encodes for the 3-phosphoshikimate 1-carboxyvinyltransferase.
The encoded enzyme is needed for the biosynthesis of essential aromatic amino acids in the shikimate pathway. It is essential in Bacillus subtilis , as three essential aromatic amino acids are products of the pathway: Trp, Phe and Tyr.
aroE encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application. An amplification of aroE allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 90
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 415
1000
COMPATIBLE WITH RFC[1000]

@@ Line 6: / Line 6: @@
 The encoded enzyme is needed for the biosynthesis of essential aromatic amino acids in the shikimate pathway. It is essential in <i>Bacillus subtilis </i>, as three essential aromatic amino acids are products of the pathway: Trp, Phe and Tyr. <br/>
 <i>aroE</i> encodes the 5-enolpyruvylshikimate-3-phosphate (EPSP) synthase, which is the main target of the herbicide glyphosate and is strongly inhibited through glyphosate application.
-<img src="https://static.igem.org/mediawiki/2018/a/a9/T--Goettingen--shikimate_pathway_ordinary.png" height="100" width="200" alt="CHIP-Logo" border="2" align="right" hspace="50" vspace="50">
 An amplification of <i>aroE</i> allows for glyphosate resistance (1), this is used in multiple GM plants to increase the yield and improve farming applications.