Difference between revisions of "Part:BBa K2586001:Design"

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===Design Notes===
 
===Design Notes===
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This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into the ''EcoRI'' and ''PstI'' digested '''pSB1C3''' backbone.
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This part was amplified from ''<i>Bacillus subtilis</i>'' genomic DNA and ligated into the ''Eco''RI and ''Pst''I digested '''pSB1C3''' backbone.
  
 
===Source===
 
===Source===

Revision as of 06:44, 11 September 2018


GltT: glutamate and glyphosate transporter in B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 742
    Illegal NgoMIV site found at 1123
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.

Source

This basic part consists of parts derived from PCR amplification of wild type B. subtilis chromosomal DNA (strain B. subtilis 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).

References