Difference between revisions of "Part:BBa K2573000"

Revision as of 20:33, 10 September 2018

MetE Coding Device

Usage and Biology

E. coli MetE codes for an enzyme that catalyzes the final step of de novo methionine biosynthesis without using an intermediate methyl carrier and in the absence of vitamin B12 (N5-methyl-H4-folate:homocysteine methyltransferasecatalyzes). Methionine is an essential amino acid required for E.coli growth.

• IMAGE* https://www.researchgate.net/figure/Biosynthesis-of-methionine-A-Methionine-synthase-MetH-MetE-reaction-B_fig8_276507951

A 2.4kb fragment containing the MetE gene from plasmid pSK397 was cloned into bba_J4450. This recombinant molecule was used to transform E.coli strain DH5 alpha, a metE mutant, to restore the MetE+ phenotype.

CHARACTERIZATION: By introducing the MetE gene into plasmid desired for insert it can be used to differentiate between E.coli cells that have successfully taken up the insert and those that have not in a medium lacking methionine.

REFERENCES: https://journals.plos.org/plosbiology/article?id=10.1371/journal.pbio.0030031 https://biocyc.org/gene?orgid=ECOLI&id=EG10584

Characterization of MetE Coding Device

Sequence and Features