Difference between revisions of "Part:BBa K2586002:Design"

 
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===Design Notes===
 
===Design Notes===
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GltP is a glutamate uptake transporter with a lower affinity to glyphosate than the other found glyphosate transporter GltT. A comparison and characterization of both transporters can lead to further understanding the influence of herbicides/antibiotics on bacteria.  
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This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into the ''EcoRI'' and ''PstI'' digested '''pSB1C3''' backbone.
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===Source===
 
===Source===
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This basic part consists of parts derived from PCR amplification of wild type ''B. subtilis'' chromosomal DNA (strain ''B. subtilis'' 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).
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The abscence of GltT causes the bacteria to develop a resistance against glyphosate, as less of this herbicide and antibiotic are transported into the cell. This means that this part has the potential to be involved in the development of glyphosate resistant detection and inactivation systems.
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===References===
 
===References===

Latest revision as of 11:00, 10 September 2018


GltP: glutamate and glyphosate uptake transporter in Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1074
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 709


Design Notes

This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.

Source

This basic part consists of parts derived from PCR amplification of wild type B. subtilis chromosomal DNA (strain B. subtilis 168; Laboratory collection AG Stülke, Department for General Microbiology, University Göttingen).

References