Difference between revisions of "Part:BBa K2586001:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part | + | This part was amplified from ''Bacillus subtilis'' genomic DNA and ligated into the ''EcoRI'' and ''PstI'' digested '''pSB1C3''' backbone. |
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===Source=== | ===Source=== | ||
Revision as of 10:12, 10 September 2018
GltT: glutamate and glyphosate transporter in B. subtilis
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 742
Illegal NgoMIV site found at 1123 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part was amplified from Bacillus subtilis genomic DNA and ligated into the EcoRI and PstI digested pSB1C3 backbone.
Source
This part is a PCR product made through de novo synthesis in the lab.