Difference between revisions of "Part:BBa I757007"
| Line 9: | Line 9: | ||
* NgoMIV / AgeI protein fusion part | * NgoMIV / AgeI protein fusion part | ||
* iGEM Team Freiburg 2007 | * iGEM Team Freiburg 2007 | ||
| − | * synthetic DNA by GeneArt | + | * synthetic DNA by GeneArt optimized for E.coli |
* Part in pGA4 vector (AmpR, ColEI ori) | * Part in pGA4 vector (AmpR, ColEI ori) | ||
Revision as of 16:10, 1 November 2007
phyA1-406_N_part
- PhyA is an interaction partner of Fhy1 (parts BBa_I757002, I757004)
- Purpose: design of a light switchable interaction
- this part codes for Amino acids 1-406, encompassing the potential Fhy1-interaction domain
- N-terminus of this protein should not be fused for fusions to avoid disturbance of folding and potential Fhy1 interaction ("N-part")
- SwissProt: P14712
- NgoMIV / AgeI protein fusion part
- iGEM Team Freiburg 2007
- synthetic DNA by GeneArt optimized for E.coli
- Part in pGA4 vector (AmpR, ColEI ori)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1219
- 1000COMPATIBLE WITH RFC[1000]