Difference between revisions of "Part:BBa I757004"

Line 7: Line 7:
 
* Purpose: design of a light switchable interaction
 
* Purpose: design of a light switchable interaction
 
* NgoMIV / AgeI protein fusion part
 
* NgoMIV / AgeI protein fusion part
# Synthetic DNA by GeneArt optimized for E.coli
+
* Synthetic DNA by GeneArt optimized for E.coli
# Part in pGA4 vector (AmpR, ColEI ori)
+
* Part in pGA4 vector (AmpR, ColEI ori)
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 16:09, 1 November 2007

Fhy1_120-202_FusionPart

  • Fhy1 is an interaction partner of PhyA
  • this part codes for Amino acids 120-202, encompassing the potential interaction domain with PhyA and additional N-terminal amino acids
  • SwissProt: Q8W565 , backtranslated
  • Purpose: design of a light switchable interaction
  • NgoMIV / AgeI protein fusion part
  • Synthetic DNA by GeneArt optimized for E.coli
  • Part in pGA4 vector (AmpR, ColEI ori)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 259
  • 1000
    COMPATIBLE WITH RFC[1000]