Difference between revisions of "Part:BBa I757003"
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# calmodulin: undergoes a strong conformational change upon binding calcium | # calmodulin: undergoes a strong conformational change upon binding calcium | ||
# purpose: design of Ca<sup>2+</sup> sensors | # purpose: design of Ca<sup>2+</sup> sensors | ||
| − | # this module comprises | + | # this module comprises xenopus N-terminal part of CaM, rat CKK binding region, xenopus C-terminal CaM |
# backtranslated from the design of K. Truong et al. Methods Mol Biol. 2007;352:71-82, Nat Struct Biol. 2001 Dec;8(12):1069-73. | # backtranslated from the design of K. Truong et al. Methods Mol Biol. 2007;352:71-82, Nat Struct Biol. 2001 Dec;8(12):1069-73. | ||
# NgoMIV / AgeI protein fusion part | # NgoMIV / AgeI protein fusion part | ||
# iGEM Team Freiburg 2007 | # iGEM Team Freiburg 2007 | ||
| − | # Synthetic DNA by GeneArt | + | # Synthetic DNA by GeneArt optimized for E.coli |
# Part in pGA4 vector (AmpR, ColEI ori) | # Part in pGA4 vector (AmpR, ColEI ori) | ||
Latest revision as of 16:08, 1 November 2007
xCaM_rCKK_xCaM_fusionpart
- calmodulin: undergoes a strong conformational change upon binding calcium
- purpose: design of Ca2+ sensors
- this module comprises xenopus N-terminal part of CaM, rat CKK binding region, xenopus C-terminal CaM
- backtranslated from the design of K. Truong et al. Methods Mol Biol. 2007;352:71-82, Nat Struct Biol. 2001 Dec;8(12):1069-73.
- NgoMIV / AgeI protein fusion part
- iGEM Team Freiburg 2007
- Synthetic DNA by GeneArt optimized for E.coli
- Part in pGA4 vector (AmpR, ColEI ori)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 4
Illegal AgeI site found at 544 - 1000COMPATIBLE WITH RFC[1000]