Difference between revisions of "Part:BBa I757003"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_I757003 short</partinfo>
 
<partinfo>BBa_I757003 short</partinfo>
  
 
# calmodulin: undergoes a strong conformational change upon binding calcium
 
# calmodulin: undergoes a strong conformational change upon binding calcium
 +
# purpose: design of Ca<sup>2+</sup> sensors
 +
# this module comprises Xenopus N-terminal part of CaM, rat CKK binding region, Xenopus C-terminal CaM
 +
# backtranslated from the design of K. Truong et al. Methods Mol Biol. 2007;352:71-82, Nat Struct Biol. 2001 Dec;8(12):1069-73. 
 
#  NgoMIV / AgeI protein fusion part
 
#  NgoMIV / AgeI protein fusion part
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# iGEM Team Freiburg 2007  
+
# iGEM Team Freiburg 2007
 +
# Synthetic DNA by GeneArt
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# Part in pGA4 vector (AmpR, ColEI ori)
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:29, 1 November 2007

xCaM_rCKK_xCaM_fusionpart

  1. calmodulin: undergoes a strong conformational change upon binding calcium
  2. purpose: design of Ca2+ sensors
  3. this module comprises Xenopus N-terminal part of CaM, rat CKK binding region, Xenopus C-terminal CaM
  4. backtranslated from the design of K. Truong et al. Methods Mol Biol. 2007;352:71-82, Nat Struct Biol. 2001 Dec;8(12):1069-73.
  5. NgoMIV / AgeI protein fusion part
  6. iGEM Team Freiburg 2007
  7. Synthetic DNA by GeneArt
  8. Part in pGA4 vector (AmpR, ColEI ori)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4
    Illegal AgeI site found at 544
  • 1000
    COMPATIBLE WITH RFC[1000]