Difference between revisions of "Part:BBa I759027"

Latest revision as of 04:33, 27 October 2007

cis3-repressed, tet-regulated Q

This part has the Q construct from I759003 with the cis repressor cis3 cloned in to replace the spacer. The weak constitutive promoter J23116 will produce a steady stream of tetR, which (in the absence of aTc) repress transcription of the pTet-cis3-Q transcript. The riboregulator design further represses levels of Q protein in the cell. The transcript will form a cis-hairpin loop and prevent translation in the absence of a riboregulator trans sequence, which binds to and opens up the cis-hairpin, allowing the ribosome to access the RBS site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 890
Illegal NheI site found at 913
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_I759027 short</partinfo>
-This part has the Q construct from I759003 with the cis repressor cis3 cloned in to replace the spacer.  The transcript will form a cis-hairpin loop and prevent translation in the absence of a riboregulator trans sequence, which binds to and opens up the cis-hairpin, allowing the ribosome to access the RBS site.
+This part has the Q construct from I759003 with the cis repressor cis3 cloned in to replace the spacer. The weak constitutive promoter J23116 will produce a steady stream of tetR, which (in the absence of aTc) repress transcription of the pTet-cis3-Q transcript. The riboregulator design further represses levels of Q protein in the cell. The transcript will form a cis-hairpin loop and prevent translation in the absence of a riboregulator trans sequence, which binds to and opens up the cis-hairpin, allowing the ribosome to access the RBS site.
+<br>
+<br>
 <!-- Add more about the biology of this part here