Difference between revisions of "Part:BBa I759027"
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<partinfo>BBa_I759027 short</partinfo> | <partinfo>BBa_I759027 short</partinfo> | ||
| − | This part has the Q construct from I759003 with the cis repressor cis3 cloned in to replace the spacer. | + | This part has the Q construct from I759003 with the cis repressor cis3 cloned in to replace the spacer. The weak constitutive promoter J23116 will produce a steady stream of tetR, which (in the absence of aTc) repress transcription of the pTet-cis3-Q transcript. The riboregulator design further represses levels of Q protein in the cell. The transcript will form a cis-hairpin loop and prevent translation in the absence of a riboregulator trans sequence, which binds to and opens up the cis-hairpin, allowing the ribosome to access the RBS site. |
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Latest revision as of 04:33, 27 October 2007
cis3-repressed, tet-regulated Q
This part has the Q construct from I759003 with the cis repressor cis3 cloned in to replace the spacer. The weak constitutive promoter J23116 will produce a steady stream of tetR, which (in the absence of aTc) repress transcription of the pTet-cis3-Q transcript. The riboregulator design further represses levels of Q protein in the cell. The transcript will form a cis-hairpin loop and prevent translation in the absence of a riboregulator trans sequence, which binds to and opens up the cis-hairpin, allowing the ribosome to access the RBS site.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 890
Illegal NheI site found at 913 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]