Difference between revisions of "Part:BBa K2810010"
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The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI. | The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI. | ||
| + | |||
| + | The [http://2018.igem.org/Team:Cardiff_Wales Cardiff_Wales] team is working with this promotor part through a collaboration with [http://2018.igem.org/Team:WashU_StLouis Washington University iGEM]. | ||
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Revision as of 09:45, 3 September 2018
Regulatory region derived from the ribitol-responsive operon
A promoter sequence that is activated by Ribitol, an ribose-derived compound. This part is GoldenGate compatible.
The promotor sequence has 5' GGAG and 3' TACT 4nt overhangs that conforms the sequence to the [http://2016.igem.org/Resources/Plant_Synthetic_Biology/PhytoBricks Phytobrick] golden gate standard. The part can be cloned into a higher order level 1 composite clone using BsaI.
The [http://2018.igem.org/Team:Cardiff_Wales Cardiff_Wales] team is working with this promotor part through a collaboration with [http://2018.igem.org/Team:WashU_StLouis Washington University iGEM].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 57
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]