Difference between revisions of "Part:BBa I759002:Design"
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===Source=== | ===Source=== | ||
− | + | Ptet was a standardized iGEM part, and the cro gene was from the lambda zap genome. Spacer-primers were specially designed so that the two parts (pTet and cro gene) could, through overlap extension PCR, be joined by the intermediary spacer. | |
===References=== | ===References=== |
Revision as of 04:05, 27 October 2007
pTet-spacer-cro
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 174
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
A pTet promoter (can be repressed by tet Repressor, and transcription activity can be restored by addition of anhydrotetracycline (aTc)) produces transcripts of a spacer (including the RBS)-cro complex. The spacer is a temporary placeholder, where later cis sequences (for cis riboregulator) will be cloned in.
Source
Ptet was a standardized iGEM part, and the cro gene was from the lambda zap genome. Spacer-primers were specially designed so that the two parts (pTet and cro gene) could, through overlap extension PCR, be joined by the intermediary spacer.