Difference between revisions of "Part:BBa K2721000:Design"
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===Source=== | ===Source=== | ||
| − | BBa_R0085 | + | The original sequence of the T7 promoter is from BBa_R0085, and we adapted PCR to induce the site mutation. |
===References=== | ===References=== | ||
Latest revision as of 12:13, 1 September 2018
Modified T7 promoter 1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This promoter is quite short. So we recommend PCR to assembly it with protein coding sequence.
Source
The original sequence of the T7 promoter is from BBa_R0085, and we adapted PCR to induce the site mutation.